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验证数据展示

  • WB analysis using 68359-1-Ig (same clone as 68359-1-PBS)

    WB analysis using 68359-1-Ig (same clone as 68359-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 68359-1-Ig (same clone as 68359-1-PBS)

    WB analysis of Jurkat using 68359-1-Ig (same clone as 68359-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

MID2 Monoclonal antibody, PBS Only

MID2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68359-1-PBS

产品说明书

CloneNo. 1F9E4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, Indirect ELISA

别名

FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68359-1-PBS targets MID2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag30793

Product name: Recombinant human MID2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 336-685 aa of BC017707

Sequence: AERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 midline 2
别名 FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1
计算分子量 715 aa, 81 kDa
观测分子量80~83 kDa
GenBank蛋白编号BC017707
基因名称 MID2
Gene ID (NCBI) 11043
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UJV3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).

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