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MID2 Monoclonal antibody

MID2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9E4

Cat No : 68359-1-Ig

Print datasheet

Synonyms

FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1

验证数据展示

  • WB analysis using 68359-1-Ig

    WB analysis using 68359-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 68359-1-Ig

    WB analysis of Jurkat using 68359-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68359-1-Ig targets MID2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen MID2 fusion protein Ag30793 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name midline 2
Synonyms FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1
Calculated Molecular Weight 715 aa, 81 kDa
Observed Molecular Weight80~83 kDa
GenBank Accession NumberBC017707
Gene Symbol MID2
Gene ID (NCBI) 11043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UJV3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).

实验方案

Product Specific Protocols
WB protocol for MID2 antibody 68359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols