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验证数据展示

  • WB analysis using 25557-1-AP

    WB analysis using 25557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25557-1-AP (LIX1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 25557-1-AP

    IF Staining of K-562 using 25557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using LIX1L antibody (25557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LIX1L Polyclonal antibody

LIX1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25557-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LIX1-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, SH-SY5Y cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25557-1-AP targets LIX1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LIX1L fusion protein Ag22209 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Lix1 homolog (mouse)-like
别名 LIX1-like protein
计算分子量 337 aa, 37 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC035727
基因名称 LIX1L
Gene ID (NCBI) 128077
RRIDAB_3669484
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Limb expression 1-like protein (LIX1L) is a putative RNA binding protein that contains a double-stranded RNA-binding motif. LIX1L is highly expressed in various cancer tissues.

实验方案

Product Specific Protocols
WB protocol for LIX1L antibody 25557-1-APDownload protocol
IF protocol for LIX1L antibody 25557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols