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验证数据展示

  • WB analysis using 18592-1-AP (same clone as 18592-1-PBS)

    WB analysis using 18592-1-AP (same clone as 18592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 18592-1-AP (same clone as 18592-1-PBS)

    IP experiment of HepG2 using 18592-1-AP (same clone as 18592-1-PBS)

    IP result of anti-FOXO1 (IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18592-1-AP (same clone as 18592-1-PBS)

    IF Staining of HeLa using 18592-1-AP (same clone as 18592-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18592-1-AP (same clone as 18592-1-PBS)

    IHC staining of human kidney using 18592-1-AP (same clone as 18592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXO1 antibody included
  • FC experiment of Daudi using 18592-1-AP (same clone as 18592-1-PBS)

    FC experiment of Daudi using 18592-1-AP (same clone as 18592-1-PBS)

    1x10^6 Daudi cells were intracellularly stained with 0.4 ug FOXO1 Polyclonal antibody (18592-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18592-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FOXO1 Polyclonal antibody, PBS Only

FOXO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 18592-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Forkhead box protein O1A, Forkhead box protein O1, forkhead box O1, FKHR, FKH1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.

产品信息

18592-1-PBS targets FOXO1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FOXO1 fusion protein Ag13296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box O1
别名 Forkhead box protein O1A, Forkhead box protein O1, forkhead box O1, FKHR, FKH1
计算分子量 655 aa, 70 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC021981
基因名称 FOXO1
Gene ID (NCBI) 2308
RRIDAB_10860103
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12778
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).