验证数据展示
经过测试的应用
| Positive WB detected in | pig cartilage tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21136-1-AP targets Fibulin-7 in WB, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, pig |
| 免疫原 |
CatNo: Ag15564 Product name: Recombinant human FBLN7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-439 aa of BC035784 Sequence: GRKFGSKYLVDHEVHFTCNPGFRLVGPSSVVCLPNGTWTGEQPHCRGISECSSQPCQNGGTCVEGVNQYRCICPPGRTGNRCQHQAQTAAPEGSVAGDSAFSRAPRCAQVERAQHCSCEAGFHLSGAAGDSVCQDVNECELYGQEGRPRLCMHACVNTPGSYRCTCPGGYRTLADGKSCEDVDECVGLQPVCPQGTTCINTGGSFQCVSPECPEGSGNVSYVKTSPFQCERNPCPMDSRPCRHLPKTISFHYLSLPSNLKTPITLFRMATASAPGRAGPNSLRFGIVGGNSRGHFVMQRSDRQTGDLILVQNLEGPQTLEVDVDMSEYLDRSFQANHVSKVTIFVSPYDF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibulin 7 |
| 别名 | FBLN7, FIBL 7, fibulin 7, TM14 |
| 计算分子量 | 439 aa, 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC035784 |
| 基因名称 | Fibulin-7 |
| Gene ID (NCBI) | 129804 |
| RRID | AB_3085638 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q53RD9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibulins are a family of extracellular matrix glycoproteins associated with basement membranes, elastic fibers, and other matrices (PMID: 19189051). The family members are defined by the presence of two structural modules, a tandem repeat of epidermal growth factor-like modules, and a unique C-terminal fibulin-type module (PMID: 19189051). They play an important role in regulating multiple cellular functions such as adhesion, growth, motility, and survival (PMID: 32429873). Fibulin-7, also named as TM14, was first identified in the mouse tooth germ cDNA microarray by differential hybridization (PMID: 17699513). Fibulin-7 is expressed in developing odontoblasts, in the giant trophoblast layer of the placenta, in the choroid of the eyes as well as in the cartilage (PMID: 32429873). It plays important roles in both the differentiation and maintenance of odontoblasts as well as in dentin formation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Fibulin-7 antibody 21136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
