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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

    WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

DMGDH Polyclonal antibody, PBS Only

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 24813-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24813-1-PBS targets DMGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DMGDH fusion protein Ag20529 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dimethylglycine dehydrogenase
别名 ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase
计算分子量 866 aa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC022388
基因名称 DMGDH
Gene ID (NCBI) 29958
RRIDAB_2879739
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI17
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.