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DIS3L2 Polyclonal antibody

DIS3L2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25792-1-AP

Print datasheet

Synonyms

DIS3 like exonuclease 2, DIS3L2, FAM6A

验证数据展示

  • IHC staining of human testis using 25792-1-AP

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 25792-1-AP

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of HepG2 using 25792-1-AP

    WB analysis of HepG2 using 25792-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25792-1-AP (DIS3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, mouse embryo tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25792-1-AP targets DIS3L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DIS3L2 fusion protein Ag22963 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DIS3 mitotic control homolog (S. cerevisiae)-like 2
Synonyms DIS3 like exonuclease 2, DIS3L2, FAM6A
Calculated Molecular Weight 885 aa, 99 kDa
Observed Molecular Weight 65 kDa, 99 kda
GenBank Accession NumberBC030113
Gene Symbol DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2880240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

实验方案

Product Specific Protocols
WB protocol for DIS3L2 antibody 25792-1-APDownload protocol
IHC protocol for DIS3L2 antibody 25792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article