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验证数据展示

  • IHC staining of human testis using 25792-1-AP

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with DIS3L2 antibody included
  • IHC staining of human testis using 25792-1-AP

    IHC staining of human testis using 25792-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25792-1-AP (DIS3L2 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with DIS3L2 antibody included
  • WB analysis of HepG2 using 25792-1-AP

    WB analysis of HepG2 using 25792-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25792-1-AP (DIS3L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

DIS3L2 Polyclonal antibody

DIS3L2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25792-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

DIS3 like exonuclease 2, DIS3L2, FAM6A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, mouse embryo tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DIS3L2 Ready-To-Use IHC Kit. DIS3L2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25792-1-AP targets DIS3L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag22963

Product name: Recombinant human DIS3L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of BC030113

Sequence: AGALGYRERLDMAPDTLQKQADHCNDSRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRIYCNALALRSHHFQKVGKKPELTLVWEPEDMEQEPAQQVITIFSLVEVVLQAEYTALKYSAILKRPGTQGHLGPEKEEEESDGEPEDSSTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DIS3 mitotic control homolog (S. cerevisiae)-like 2
别名 DIS3 like exonuclease 2, DIS3L2, FAM6A
计算分子量 885 aa, 99 kDa
观测分子量 65 kDa, 99 kda
GenBank蛋白编号BC030113
基因名称 DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2880240
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

实验方案

Product Specific Protocols
WB protocol for DIS3L2 antibody 25792-1-APDownload protocol
IHC protocol for DIS3L2 antibody 25792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article