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验证数据展示

  • WB analysis using 26980-1-AP (same clone as 26980-1-PBS)

    WB analysis using 26980-1-AP (same clone as 26980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26980-1-AP (Connexin 43 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 26980-1-AP (Connexin 43 antibody) at dilution of 1:50 and 66895-1-Ig (alpha Actinin mouse mAb) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 26980-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66895-1-Ig. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    IHC staining of rat heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IHC staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IHC staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26980-1-AP (Connexin 43 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GJA1/Connexin 43 antibody included
  • IP experiment of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IP experiment of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IP result of anti-Connexin 43 (IP:26980-1-AP, 4ug; Detection:26980-1-AP 1:500) with mouse heart tissue lysate 4000 ug. This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Connexin 43 antibody (26980-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    IF Staining of mouse heart using 26980-1-AP (same clone as 26980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Connexin 43 antibody (26980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26980-1-PBS in a different storage buffer formulation.

Connexin 43 Polyclonal antibody, PBS Only

Connexin 43 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 26980-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

GJA1, Connexin-43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy GJA1/Connexin 43 Ready-To-Use IHC Kit. GJA1/Connexin 43 primary antibody included.

产品信息

26980-1-PBS targets Connexin 43 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25533

Product name: Recombinant human Connexin 43 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 232-382 aa of BC026329

Sequence: FFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, alpha 1, 43kDa
别名 GJA1, Connexin-43, GJAL, Gap junction alpha-1 protein, Gap junction alpha 1 protein
计算分子量 43 kDa
观测分子量 43-47 kDa
GenBank蛋白编号BC026329
基因名称 Connexin-43
Gene ID (NCBI) 2697
RRIDAB_2880711
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17302
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

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