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Species Reactivity
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0
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验证数据展示

  • WB analysis of HCT 116 using 21327-1-AP (same clone as 21327-1-PBS)

    WB analysis of HCT 116 using 21327-1-AP (same clone as 21327-1-PBS)

    WB result of Cathepsin D antibody (21327-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and sg-Cathepsin D transfected HCT-116 cells. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    IHC staining of human hepatocirrhosis using 21327-1-AP (same clone as 21327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    WB analysis using 21327-1-AP (same clone as 21327-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 21327-1-AP (same clone as 21327-1-PBS)

    IF Staining of MCF-7 using 21327-1-AP (same clone as 21327-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 21327-1-AP (same clone as 21327-1-PBS)

    IF Staining of A431 using 21327-1-AP (same clone as 21327-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21327-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cathepsin D Polyclonal antibody, PBS Only

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21327-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

产品信息

21327-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Cathepsin D fusion protein Ag15254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin D
别名 CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD
计算分子量 412 aa, 45 kDa
观测分子量 32 kDa, 48 kDa, 52 kDa
GenBank蛋白编号BC016320
基因名称 Cathepsin D
Gene ID (NCBI) 1509
RRIDAB_10733646
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07339
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).