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验证数据展示

  • FC experiment of mouse splenocytes using 98147-1-RR

    FC experiment of mouse splenocytes using 98147-1-RR

    1x10^6 untreated or Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98147-1-RR

    FC experiment of mouse splenocytes using 98147-1-RR

    1x10^6 Con A treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody (98147-1-RR, Clone: 241100B8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98147-1-RR

    Affinity and Kinetic Characterization of 98147-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98147-1-RR against Mouse CTLA-4/CD152 were performed. The affinity constant is 0.580 nM.

Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98147-1-RR

产品说明书

CloneNo. 241100B8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ctla4, Ly 56, Ctla 4, Cd152, 241100B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inCon A treated mouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98147-1-RR targets CTLA-4/CD152 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytotoxic T-lymphocyte-associated protein 4
别名 Ctla4, Ly 56, Ctla 4, Cd152, 241100B8
计算分子量25 kDa
GenBank蛋白编号NM_009843.4
基因名称 CTLA-4
Gene ID (NCBI) 12477
RRIDAB_3672292
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09793
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

实验方案

Product Specific Protocols
FC protocol for CTLA-4/CD152 antibody 98147-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols