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CETP Polyclonal antibody

CETP Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13459-1-AP

Print datasheet

Synonyms

CETP, HDLCQ10, Lipid transfer protein I

验证数据展示

  • WB analysis of human brain using 13459-1-AP

    WB analysis of human brain using 13459-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13459-1-AP (CETP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13459-1-AP

    IF Staining of HepG2 using 13459-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CETP antibody (13459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13459-1-AP targets CETP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen CETP fusion protein Ag3923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cholesteryl ester transfer protein, plasma
Synonyms CETP, HDLCQ10, Lipid transfer protein I
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight 50-70, 100 kDa
GenBank Accession NumberBC025739
Gene Symbol CETP
Gene ID (NCBI) 1071
RRIDAB_2082373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cholesteryl ester transfer protein (CETP) is a plasma glycoprotein which transfers cholesteryl esters, triglycerides, and phospholipids between lipoproteins. In plasma, CETP is mainly associated with HDL particles and catalyzes the transfer of cholesteryl esters from HDL to apolipoprotein (apo) B-containing plasma lipoproteins. Low plasma concentrations of high-density lipoprotein-cholesterol (HDL-C) have long been recognized as a major, independent cardiovascular disease (CVD) risk factor. It demonstrated that altered level of plasma CETP was associated with the increased risk of CVD (PMID: 17991755, PMID: 23477743). CETP can be detected a 70 kDa isoform by glycosylation (PMID: 3281933,PMID: 22056562). It also can be detected 100 kDa dimer (PMID: 7744792).

实验方案

Product Specific Protocols
WB protocol for CETP antibody 13459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Biol Chem

Regulation of hepatic cholesteryl ester transfer protein expression and reverse cholesterol transport by inhibition of DNA topoisomerase II.

Authors - Mengyang Liu
View Article
humanIHC

J Transl Med

Characterization of the proteome of stable and unstable carotid atherosclerotic plaques using data-independent acquisition mass spectrometry

Authors - Zhichao Lai
View Article