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验证数据展示

  • WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

CERK Polyclonal antibody, PBS Only

CERK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25731-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25731-1-PBS targets CERK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22774

Product name: Recombinant human CERK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 224-537 aa of BC126940

Sequence: AVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ceramide kinase
别名
计算分子量 537 aa, 60 kDa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC126940
基因名称 CERK
Gene ID (NCBI) 64781
RRIDAB_2880214
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCT0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.

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