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验证数据展示

  • FC experiment of human peripheral blood leukocytes using PY7-FcA98507-2

    FC experiment of human peripheral blood leukocytes using PY7-FcA98507-2

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE-Cyanine7 Anti-Human CEACAM8/CD66b Rabbit RecAb (PY7-FcA98507-2, Clone: 242954D11) or unstained. Cells were incubated with MonoZero™ Monocytes blocking Reagent(PF00020) prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using PY7-FcA98507-2

    FC experiment of human peripheral blood leukocytes using PY7-FcA98507-2

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE-Cyanine7 Anti-Human CEACAM8/CD66b Rabbit RecAb (PY7-FcA98507-2, Clone: 242954D11) (red) or unstained (blue). Cells were incubated with MonoZero™ Monocytes blocking Reagent (PF00020) prior to staining. Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® PE-Cyanine7 Anti-Human CEACAM8/CD66b Rabbit Recombinant Antibody

CEACAM8/CD66b FcZero-rAb® Recombinant Antibody for FC
Cat No. PY7-FcA98507-2

产品说明书

CloneNo. 242954D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  PE-Cyanine7
规格价格库存

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PY7-FcA98507-2 targets CEACAM8/CD66b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg32133

Product name: Recombinant Human CEACAM-8 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 35-319 aa of BC026263

Sequence: QLTIEAVPSNAAEGKEVLLLVHNLPQDPRGYNWYKGETVDANRRIIGYVISNQQITPGPAYSNRETIYPNASLLMRNVTRNDTGSYTLQVIKLNLMSEEVTGQFSVHPETPKPSISSNNSNPVEDKDAVAFTCEPETQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLLSVTRNDVGPYECEIQNPASANFSDPVTLNVLYGPDAPTISPSDTYYHAGVNLNLSCHAASNPPSQYSWSVNGTFQQYTQKLFIPNITTKNSGSYACHTTNSATGRNRTTVRMITVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 8
别名 CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b
计算分子量 38 kDa
GenBank蛋白编号BC026263
基因名称 CEACAM8
Gene ID (NCBI) 1088
偶联类型 PE-Cyanine7 Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 778 nm
激发激光Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31997
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).

实验方案

Product Specific Protocols
FC protocol for PE-Cyanine7 CEACAM8/CD66b antibody PY7-FcA98507-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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