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验证数据展示

  • WB analysis using 30380-1-AP (same clone as 30380-1-PBS)

    WB analysis using 30380-1-AP (same clone as 30380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30380-1-AP (CDX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of mouse colon using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of mouse colon using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of rat colon using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of rat colon using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 30380-1-AP (CDX2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    IHC staining of human colon cancer using 30380-1-AP (same clone as 30380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 30380-1-AP (same clone as 30380-1-PBS)

    IF Staining of Caco-2 using 30380-1-AP (same clone as 30380-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (30380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 30380-1-PBS in a different storage buffer formulation.

CDX2 Polyclonal antibody, PBS Only

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30380-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CDX3, CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

产品信息

30380-1-PBS targets CDX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag32826

Product name: Recombinant human CDX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC014461

Sequence: MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caudal type homeobox 2
别名 CDX3, CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2
计算分子量 313 aa, 34 kDa
观测分子量 33-40 kDa
GenBank蛋白编号BC014461
基因名称 CDX2
Gene ID (NCBI) 1045
RRIDAB_3086303
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99626
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

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