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验证数据展示

  • WB analysis using 87542-1-RR

    WB analysis using 87542-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87542-1-RR (ITAL antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 87542-1-RR

    IHC staining of human tonsillitis using 87542-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87542-1-RR (CD11a antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 87542-1-RR

    IF Staining of RAW 264.7 using 87542-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using CD11a antibody (87542-1-RR, Clone: 252754D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD11a Recombinant monoclonal antibody

CD11a Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87542-1-RR

产品说明书

CloneNo. 252754D9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Itgal, integrin alpha L, LFA 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87542-1-RR targets CD11a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg10464

Product name: Recombinant mouse ITAL protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011451 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin alpha L
别名 Itgal, integrin alpha L, LFA 1
观测分子量180 kDa
基因名称 CD11a
Gene ID (NCBI) 16408
RRIDAB_3745611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDB7ZN92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
IF protocol for CD11a antibody 87542-1-RRDownload protocol
IHC protocol for CD11a antibody 87542-1-RRDownload protocol
WB protocol for CD11a antibody 87542-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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