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验证数据展示

  • WB analysis using 87542-1-RR (same clone as 87542-1-PBS)

    WB analysis using 87542-1-RR (same clone as 87542-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87542-1-RR (ITAL antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 87542-1-RR (same clone as 87542-1-PBS)

    IHC staining of human tonsillitis using 87542-1-RR (same clone as 87542-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87542-1-RR (CD11a antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 87542-1-RR (same clone as 87542-1-PBS)

    IF Staining of RAW 264.7 using 87542-1-RR (same clone as 87542-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using CD11a antibody (87542-1-RR, Clone: 252754D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87542-1-PBS in a different storage buffer formulation.

Mouse CD11a Recombinant monoclonal antibody, PBS Only

CD11a Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87542-1-PBS

产品说明书

CloneNo. 252754D9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Itgal, integrin alpha L, LFA 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87542-1-PBS targets CD11a in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg10464

Product name: Recombinant mouse ITAL protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011451 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin alpha L
别名 Itgal, integrin alpha L, LFA 1
观测分子量180 kDa
基因名称 CD11a
Gene ID (NCBI) 16408
RRIDAB_3745611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDB7ZN92
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

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