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CCT2 Monoclonal antibody

CCT2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rabbit, Rat and More (1)

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H6C7

Cat No : 68214-1-Ig

Print datasheet

Synonyms

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

验证数据展示

  • WB analysis using 68214-1-Ig

    WB analysis using 68214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of NIH/3T3 using 68214-1-Ig

    IF Staining of NIH/3T3 using 68214-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IF detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68214-1-Ig targets CCT2 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Pig, Rabbit, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Pig, Rabbit, Rat
Cited Reactivitymouse
Immunogen CCT2 fusion protein Ag19264 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 2 (beta)
Synonyms 99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta
Calculated Molecular Weight 535 aa, 57 kDa
Observed Molecular Weight 50-58 kDa
GenBank Accession NumberBC113516
Gene Symbol CCT2
Gene ID (NCBI) 10576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

实验方案

Product Specific Protocols
WB protocol for CCT2 antibody 68214-1-IgDownload protocol
IF protocol for CCT2 antibody 68214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins

Authors - Joshua Linnert
View Article