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验证数据展示

  • WB analysis using 26500-1-AP

    WB analysis using 26500-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26500-1-AP (CCDC134 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

CCDC134 Polyclonal antibody

CCDC134 Polyclonal Antibody for WB, ELISA
Cat No. 26500-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, hamster

应用

WB, ELISA

别名

CCDC134

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, CHO cells
Planning an IHC experiment? We recommend our IHCeasy CCDC134 Ready-To-Use IHC Kit. CCDC134 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26500-1-AP targets CCDC134 in WB, ELISA applications and shows reactivity with human, hamster samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, hamster
免疫原

CatNo: Ag21990

Product name: Recombinant human CCDC134 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-229 aa of BC017693

Sequence: TLRTSLDPSLEIYKKMFEVKRREQLLALKNLAQLNDIHQQYKILDVMLKGLFKVLEDSRTVLTAADVLPDGPFPQDEKLKDAFSHVVENTAFFGDVVLRFPRIVHYYFDHNSNWNLLIRWGISFCNQTGVFNQGPHSPILSLMAQELGISEKDSNFQNPFKIDRTEFIPSTDPFQKALREEEKRRKKEEKRKEIRKGPRISRSQSEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 134
别名 CCDC134
计算分子量 229 aa, 27 kDa
观测分子量27 kda
GenBank蛋白编号BC017693
基因名称 CCDC134
Gene ID (NCBI) 79879
RRIDAB_3669541
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6E4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC134 (coiled-coil domain containing 134) encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein (PMID: 18087676). CCDC134 is in extracellular secreted form, promotes proliferation and activation of CD8(+) T cells, suggesting a cytokine-like function (PubMed: 25125657). CCDC134 is widely expressed in normal adult tissues, tumor tissues and cell lines with the molecular weight of 27 kDa.

实验方案

Product Specific Protocols
WB protocol for CCDC134 antibody 26500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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