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验证数据展示

  • WB analysis of A431 using 85736-6-RR (same clone as 85736-6-PBS)

    WB analysis of A431 using 85736-6-RR (same clone as 85736-6-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 85736-6-RR (C1S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85736-6-PBS in a different storage buffer formulation.

  • WB analysis using 85736-6-RR (same clone as 85736-6-PBS)

    WB analysis using 85736-6-RR (same clone as 85736-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85736-6-RR (C1S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85736-6-PBS in a different storage buffer formulation.

  • Affinity of 85736-6-RR

    Affinity of 85736-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85736-6-RR against Human C1S were performed. The affinity constant is 1.63 nM.

C1S Recombinant monoclonal antibody, PBS Only

C1S Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85736-6-PBS

产品说明书

CloneNo. 250031G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

Complement C1s, C1 esterase, Complement C1s subcomponent, EC:3.4.21.42

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy C1S Ready-To-Use IHC Kit. C1S primary antibody included.

产品信息

85736-6-PBS targets C1S in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5977

Product name: Recombinant human C1S protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-688 aa of BC056903

Sequence: DDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 1, s subcomponent
别名 Complement C1s, C1 esterase, Complement C1s subcomponent, EC:3.4.21.42
计算分子量 77 kDa
观测分子量 88 kDa
GenBank蛋白编号BC056903
基因名称 C1S
Gene ID (NCBI) 716
RRIDAB_3744333
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09871
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

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