验证数据展示
经过测试的应用
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells, mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85871-4-RR targets Beta-2-Microglobulin in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg3086 Product name: Recombinant Mouse Beta-2-Microglobulin protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-119 aa of NM_009735.3 Sequence: IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | beta-2 microglobulin |
| 别名 | B2m, beta 2 microglobulin |
| 计算分子量 | 14 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | NM_009735.3 |
| 基因名称 | B2m |
| Gene ID (NCBI) | 12010 |
| RRID | AB_3744435 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01887 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta-2-Microglobulin antibody 85871-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
