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验证数据展示

  • WB analysis using 85871-4-RR (same clone as 85871-4-PBS)

    WB analysis using 85871-4-RR (same clone as 85871-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85871-4-RR (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85871-4-PBS in a different storage buffer formulation.

  • Affinity of 85871-4-RR

    Affinity of 85871-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85871-4-RR against Mouse Beta-2-Microglobulin were performed. The affinity constant is 18.9 nM.

Mouse Beta-2-Microglobulin Recombinant monoclonal antibody, PBS Only

Beta-2-Microglobulin Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85871-4-PBS

产品说明书

CloneNo. 250172E4

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, Indirect ELISA

别名

B2m, beta 2 microglobulin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85871-4-PBS targets Beta-2-Microglobulin in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3086

Product name: Recombinant Mouse Beta-2-Microglobulin protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-119 aa of NM_009735.3

Sequence: IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 beta-2 microglobulin
别名 B2m, beta 2 microglobulin
计算分子量14 kDa
观测分子量 12 kDa
GenBank蛋白编号NM_009735.3
基因名称 B2m
Gene ID (NCBI) 12010
RRIDAB_3744435
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01887
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

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