ATP6V1F Polyclonal antibody
ATP6V1F Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human testis tissue, human brain tissue, human kidney tissue, human pancreas tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17725-1-AP targets ATP6V1F in WB, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IHC, WB,ELISA |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | ATP6V1F fusion protein Ag12121 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F |
| Synonyms | ATP6S14, ATP6V1F, V ATPase 14 kDa subunit, V ATPase subunit F, V type proton ATPase subunit F, Vacuolar proton pump subunit F, VATF, Vma7 |
| Calculated Molecular Weight | 119 aa, 13 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC107854 |
| Gene Symbol | ATP6V1F |
| Gene ID (NCBI) | 9296 |
| RRID | AB_2062680 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16864 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V1F antibody 17725-1-AP | Download protocol |
| IHC protocol for ATP6V1F antibody 17725-1-AP | Download protocol |
| FC protocol for ATP6V1F antibody 17725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Trace Elem Med Biol Study on the mechanism of arsenic-induced renal injury based on SWATH proteomics technology | ||
BMC Med Genomics ATP6V1F is a novel prognostic biomarker and potential immunotherapy target for hepatocellular carcinoma | ||
J Proteome Res Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia | ||
Front Oncol Genome-wide association studies identify miRNA-194 as a prognostic biomarker for gastrointestinal cancer by targeting ATP6V1F, PPP1R14B, BTF3L4 and SLC7A5 |
