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验证数据展示

  • WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

    WB analysis using 66643-1-Ig (same clone as 66643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66643-1-Ig (ANLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

    WB analysis of MCF-7 using 66643-1-Ig (same clone as 66643-1-PBS)

    WB result of ANLN antibody (66643-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANLN transfected MCF-7 cells. This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66643-1-Ig (same clone as 66643-1-PBS)

    IF Staining of A431 using 66643-1-Ig (same clone as 66643-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANLN antibody (66643-1-Ig, Clone: 1B4A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66643-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ANLN Monoclonal antibody, PBS Only

ANLN Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66643-1-PBS

产品说明书

CloneNo. 1B4A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66643-1-PBS targets ANLN in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ANLN fusion protein Ag19294 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 anillin, actin binding protein
别名 Anillin, Scraps, scra, anillin, actin binding protein, Actin binding protein anillin
计算分子量 1124 aa, 124 kDa
观测分子量 110 kDa~120 kDa
GenBank蛋白编号BC034692
基因名称 ANLN
Gene ID (NCBI) 54443
RRIDAB_2815000
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NQW6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Anillin, an actin-binding protein, is a conserved cleavage furrow component important for cytokinesis. The human anillin protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. It interactions with at least two other furrow proteins, actin and the septins through its C-terminal PH domain and N-terminal actin-binding domain. In anillin knockdown cells, the cleavage furrow ingressed but failed to complete the ingression. Anillin has been found to be overexpressed in tumors and may serve as promising marker for pancreatic carcinoma molecular diagnostics as well as a prognostic indicator for non-small cell lung cancers.