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验证数据展示

  • WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    WB analysis of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14631-1-AP (same clone as 14631-1-PBS)

    IF Staining of HepG2 using 14631-1-AP (same clone as 14631-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    IP experiment of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AFG3L2 Polyclonal antibody, PBS Only

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14631-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14631-1-PBS targets AFG3L2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6209

Product name: Recombinant human AFG3L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 449-797 aa of BC065016

Sequence: ILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHLRPLKLDSTLEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AFG3 ATPase family gene 3-like 2 (yeast)
别名
计算分子量 88 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC065016
基因名称 AFG3L2
Gene ID (NCBI) 10939
RRIDAB_2242420
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4W6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

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