Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

Various lysates were subjected to SDS PAGE followed by western blot with 81323-2-RR (VEGFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human VEGF165 (HZ-1038) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.