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- KD/KO Validated
VEGFA Polyclonal antibody
VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (6)
Applications
FC, IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
582
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | NIH/3T3 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
19003-1-AP targets VEGFA in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | IF, IHC, IP, RIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat, sheep, monkey, rabbit, pig |
| Immunogen | VEGFA fusion protein Ag13500 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vascular endothelial growth factor A |
| Synonyms | L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF |
| Calculated molecular weight | 412 aa, 46 kDa |
| Observed molecular weight | 36-40 kDa |
| GenBank accession number | BC065522 |
| Gene symbol | VEGFA |
| Uniprot | P15692 |
| Gene ID (NCBI) | 7422 |
| RRID | AB_2212657 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VEGFA antibody 19003-1-AP | Download protocol |
| IHC protocol for VEGFA antibody 19003-1-AP | Download protocol |
| FC protocol for VEGFA antibody 19003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.
| ||
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Light Sci Appl Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model. | ||
J Clin Invest Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema. | ||
Bone Res Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation | ||
Redox Biol Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy |
