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- KD/KO Validated
VEGFA Polyclonal antibody
VEGFA Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (6)
Applications
FC, IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
582
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | NIH/3T3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
19003-1-AP targets VEGFA in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IHC, WB, ELISA |
Cited Applications | IF, IHC, IP, RIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, goat, sheep, monkey, rabbit, pig |
Immunogen | VEGFA fusion protein Ag13500 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | vascular endothelial growth factor A |
Synonyms | L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF |
Calculated molecular weight | 412 aa, 46 kDa |
Observed molecular weight | 36-40 kDa |
GenBank accession number | BC065522 |
Gene symbol | VEGFA |
Uniprot | P15692 |
Gene ID (NCBI) | 7422 |
RRID | AB_2212657 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for VEGFA antibody 19003-1-AP | Download protocol |
IHC protocol for VEGFA antibody 19003-1-AP | Download protocol |
FC protocol for VEGFA antibody 19003-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.
| ||
ACS Nano Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA. | ||
Light Sci Appl Microglia modulation with 1070-nm light attenuates Aβ burden and cognitive impairment in Alzheimer's disease mouse model. | ||
J Clin Invest Angiopoietin-like 4 binds neuropilins and cooperates with VEGF to induce diabetic macular edema. | ||
Bone Res Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation | ||
Redox Biol Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy |