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验证数据展示

  • WB analysis using 19003-1-AP

    WB analysis using 19003-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 19003-1-AP

    IHC staining of human lung cancer using 19003-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • IF Staining of HeLa using 19003-1-AP

    IF Staining of HeLa using 19003-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human lung cancer using 19003-1-AP

    IHC staining of human lung cancer using 19003-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19003-1-AP (VEGF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • FC experiment of NIH/3T3 using 19003-1-AP

    FC experiment of NIH/3T3 using 19003-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human VEGFA (19003-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 19003-1-AP

    WB analysis using 19003-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19003-1-AP (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 19003-1-AP

    IP experiment of Jurkat using 19003-1-AP

    IP result of anti-VEGFA (IP:19003-1-AP, 4ug; Detection:19003-1-AP 1:3000) with Jurkat cells lysate 1560 ug.

  • IF Staining of HeLa using 19003-1-AP

    IF Staining of HeLa using 19003-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VEGFA antibody (19003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

VEGFA Polyclonal antibody

VEGFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 19003-1-AP

产品说明书

引用文献(851)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (7)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

VEGF, L-VEGF, N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, C6 cells, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19003-1-AP targets VEGFA in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, rabbit, monkey, bovine, goat, sheep
免疫原 VEGFA fusion protein Ag13500 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, L-VEGF, N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A
计算分子量 412 aa, 46 kDa
观测分子量36-40 kDa
GenBank蛋白编号BC065522
基因名称 VEGFA
Gene ID (NCBI) 7422
RRIDAB_2212657
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 19003-1-APDownload protocol
IHC protocol for VEGFA antibody 19003-1-APDownload protocol
IF protocol for VEGFA antibody 19003-1-APDownload protocol
IP protocol for VEGFA antibody 19003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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