HepG2 cells were subjected to SDS PAGE followed by western blot with 26846-1-AP (TRAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26846-1-AP (TRAF2 Antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (4% PFA) fixed A549 cells using TRAF2 antibody (26846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®488-conjugated TRAF2 antibody (CL488-67315, Clone: 2A4D1 ) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human TRAF2 (CL488-67315, Clone:2A4D1) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TRAF2 antibody (CL594-67315, Clone: 2A4D1 ) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TRAF2 (CL594-67315, Clone:2A4D1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67315-1-PBS in a different storage buffer formulation.

THP-1 cells were subjected to SDS PAGE followed by western blot with 27065-1-AP (TANK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

TIFA fusion protein Ag32572 SDS-PAGE

TIFA fusion protein Ag1876 SDS-PAGE