Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BAD (10435-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 10x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAD antibody (67830-1-Ig, Clone: 4B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAD antibody (67830-1-Ig, Clone: 4B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 BAD antibody (CL488-67830, Clone: 4B12G2 ) at dilution of 1:200.

PIM2 antibody (mAb) tested by Immunohistochemistry PIM2 detected by immunohistochemistry in paraffin-embedded human tonsil cells.

PIM2 antibody (mAb) tested by Western blot Ramos cell extract (100 ug per lane) probed with PIM2 antibody (mAb).

Recombinant PIM2 protein 10% SDS-PAGE with Coomassie staining MW: 35.5 kDa Purity: >95%

HTRF assay for PIM2 activity 1 uM STK S3 substrate was incubated with different concentrations of PIM2 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

HTRF assay for PIM2 activity 1 uM STK S3 substrate was incubated with different concentrations of PIM2 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.