验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20988-1-AP targets Hamartin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tuberous sclerosis 1 |
| 别名 | Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein |
| 计算分子量 | 130 kDa |
| 观测分子量 | |
| GenBank蛋白编号 | NM_000368 |
| 基因名称 | Hamartin/TSC1 |
| Gene ID (NCBI) | 7248 |
| RRID | AB_10950398 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92574 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC1, also named as KIAA0243 and TSC, is implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. In complex with TSC2, TSC1 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It seems not to be required for TSC2 GAP activity towards RHEB. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). The antibody is specific to TSC1. This antibody can recognize two isoforms: 70 kDa (667aa) and 130 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Hamartin antibody 20988-1-AP | Download protocol |
| IF protocol for Hamartin antibody 20988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells. | ||
Theranostics Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.
| ||
Mol Ther Nucleic Acids miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway.
| ||
Sci Rep Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.
| ||
Biochem Biophys Res Commun UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1.
|
