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验证数据展示

  • WB analysis of HeLa using 23163-1-AP

    WB analysis of HeLa using 23163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23163-1-AP (ZFYVE19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23163-1-AP

    IHC staining of human liver cancer using 23163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23163-1-AP (ZFYVE19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZFYVE19 Polyclonal antibody

ZFYVE19 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23163-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Abscission/NoCut checkpoint regulator, ANCHR, MPFYVE, Zinc finger FYVE domain-containing protein 19

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23163-1-AP targets ZFYVE19 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19593

Product name: Recombinant human ZFYVE19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-471 aa of BC015738

Sequence: VCKQCHEVLTRGSSANASKWSPPQNYKKRVAALEAKQKPSTSQSQGLTRQDQMIAERLARLRQENKPKLVPSQAEIEARLAALKDERQGSIPSTQEMEARLAALQGRVLPSQTPQPAHHTPDTRTQAQQTQDLLTQLAAEVAIDESWKGGGPAASLQNDLNQGGPGSTNSKRQANWSLEEEKSRLLAEAALELREENTRQERILALAKRLAMLRGQDPERVTLQDYRLPDSDDDEDEETAIQRVLQQLTEEAALDEASGFNIPAEQASRPWTQPRGAEPEAQDVDPRPEAEEEELPWCCICNEDATLRCAGCDGDLFCARCFREGHDAFELKEHQTSAYSPPRAGQEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 19
别名 Abscission/NoCut checkpoint regulator, ANCHR, MPFYVE, Zinc finger FYVE domain-containing protein 19
计算分子量 471 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC015738
基因名称 ZFYVE19
Gene ID (NCBI) 84936
RRIDAB_2879219
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZFYVE19, also known as ANCHR, is a poorly characterized human protein. Recent clinical studies have linked biallelic loss-of-function mutations to a progressive familial intrahepatic cholestasis (PFIC) phenotype featuring neonatal-onset high-GGT cholestasis, congenital hepatic fibrosis, and sclerosing cholangiopathy. (PMID: 33853651)

实验方案

Product Specific Protocols
IHC protocol for ZFYVE19 antibody 23163-1-APDownload protocol
WB protocol for ZFYVE19 antibody 23163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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