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ZC3H8 Monoclonal antibody

ZC3H8 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C6

Cat No : 68282-1-Ig

Print datasheet

Synonyms

Fliz1, ZC3H8, ZC3HDC8

验证数据展示

  • WB analysis using 68282-1-Ig

    WB analysis using 68282-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68282-1-Ig targets ZC3H8 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen ZC3H8 fusion protein Ag23496 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type containing 8
Synonyms Fliz1, ZC3H8, ZC3HDC8
Observed Molecular Weight36 kDa
GenBank Accession NumberBC032001
Gene Symbol ZC3H8
Gene ID (NCBI) 84524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5P1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).

实验方案

Product Specific Protocols
WB protocol for ZC3H8 antibody 68282-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols