验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, HepG2 cells, human skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
产品信息
14945-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6747 Product name: Recombinant human ZAK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-312 aa of BC001401 Sequence: MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterile alpha motif and leucine zipper containing kinase AZK |
| 别名 | AZK, HCCS 4, MAP3K20, Mitogen-activated protein kinase kinase kinase 20, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK |
| 计算分子量 | 91 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC001401 |
| 基因名称 | ZAK |
| Gene ID (NCBI) | 51776 |
| RRID | AB_10642698 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYL2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZAK antibody 14945-1-AP | Download protocol |
| IHC protocol for ZAK antibody 14945-1-AP | Download protocol |
| WB protocol for ZAK antibody 14945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.
| ||
Sci Signal Poly-GR repeats associated with ALS/FTD gene C9ORF72 impair translation elongation and induce a ribotoxic stress response in neurons | ||
bioRxiv Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L | ||
PNAS Nexus Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability | ||
Mol Cell The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo | ||
Nat Commun Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation |
