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验证数据展示

  • IHC staining of mouse spleen using 33408-1-AP

    IHC staining of mouse spleen using 33408-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 33408-1-AP

    IHC staining of mouse spleen using 33408-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33408-1-AP (XCL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XCL1 Polyclonal antibody

XCL1 Polyclonal Antibody for IHC, ELISA
Cat No. 33408-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, ELISA

别名

C motif chemokine 1, Cytokine SCM-1, Lptn, Ltn, Lymphotactin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse spleen tissue, mouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33408-1-AP targets XCL1 in IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2932

Product name: recombinant mouse Xcl1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 22-114 aa of NM_008510.1

Sequence: VGTEVLEESSCVNLQTQRLPVQKIKTYIIWEGAMRAVIFVTKRGLKICADPEAKWVKAAIKTVDGRASTRKNMAETVPTGAQRSTSTAITLTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C motif) ligand 1
别名 C motif chemokine 1, Cytokine SCM-1, Lptn, Ltn, Lymphotactin
计算分子量12 kDa
GenBank蛋白编号NM_008510.1
基因名称 Xcl1
Gene ID (NCBI) 16963
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP47993
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XCL1/lymphotactin is a member of the chemokine family, which is expressed by immune cells, including activated CD8+ T cells, CD4‏+ T cells, natural killer cells (NKCs), and natural killer T (NKT) cells. XCR1, the XCL1 receptor, is a member of the C-chemokine subfamily expressed on CD4+ and CD8+ T cells, NKCs, B cells, and neutrophils. XCL1 has a potential role in inflammatory diseases, including chronic inflammatory bowel disease, autoimmune diabetes, encephalomyelitis, and rheumatoid arthritis (RA).

实验方案

Product Specific Protocols
IHC protocol for XCL1 antibody 33408-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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