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验证数据展示

  • WB analysis using 68361-1-Ig

    WB analysis using 68361-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68361-1-Ig (BAZ1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WSTF Monoclonal antibody

WSTF Monoclonal Antibody for WB, ELISA
Cat No. 68361-1-Ig

产品说明书

CloneNo. 2F9F12

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

BAZ1B, hWALp2, Tyrosine protein kinase BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, T-47D cells, MDA-MB-231 cells, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy® BAZ1B/WSTF Ready-To-Use IHC Kit. BAZ1B/WSTF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68361-1-Ig targets WSTF in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag24403

Product name: Recombinant human BAZ1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1144-1483 aa of BC136520

Sequence: KVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bromodomain adjacent to zinc finger domain, 1B
别名 BAZ1B, hWALp2, Tyrosine protein kinase BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF
计算分子量 1483 aa, 171 kDa
观测分子量170 kDa
GenBank蛋白编号BC136520
基因名称 WSTF/BAZ1B
Gene ID (NCBI) 9031
RRIDAB_3085083
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UIG0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosine-protein kinase BAZ1B, also named WSTF, plays a central role in chromatin remodeling and acts as a transcription regulator (PMID:19092802). It involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph) (PMID:19234442).

实验方案

Product Specific Protocols
WB protocol for WSTF antibody 68361-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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