验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
67452-1-Ig targets WNT3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag27957 Product name: Recombinant human WNT3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 130-306 aa of BC114219 Sequence: SCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYSLFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | wingless-type MMTV integration site family, member 3 |
| 别名 | INT4, Proto oncogene Int 4 homolog, Proto oncogene Wnt 3, WNT3 |
| 计算分子量 | 355 aa, 40 kDa |
| GenBank蛋白编号 | BC114219 |
| 基因名称 | WNT3 |
| Gene ID (NCBI) | 7473 |
| RRID | AB_2882686 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P56703 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WNT3 antibody 67452-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int miR-454 suppresses the proliferation and invasion of ovarian cancer by targeting E2F6. | ||
Chin J Integr Med Babao Dan () Alleviates 5-Fluorouracil-Induced Intestinal Damage via Wnt/β-Catenin Pathway. | ||
Kidney Int Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis | ||
World J Gastrointest Oncol N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway |
