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验证数据展示

  • IF Staining of HEK-293 using 20374-1-AP (same clone as 20374-1-PBS)

    IF Staining of HEK-293 using 20374-1-AP (same clone as 20374-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20374-1-AP (USP50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 20374-1-AP (same clone as 20374-1-PBS)

    WB analysis of mouse brain using 20374-1-AP (same clone as 20374-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

    IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

    IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP50 Polyclonal antibody, PBS Only

USP50 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20374-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

20374-1-PBS targets USP50 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 50
别名
计算分子量 39 kDa
观测分子量 40 kDa
GenBank蛋白编号NM_203494
基因名称 USP50
Gene ID (NCBI) 373509
RRIDAB_10973680
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70EL3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function through targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.

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