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验证数据展示

  • WB analysis using 32710-1-AP

    WB analysis using 32710-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32710-1-AP (Ucp1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brown adipose using 32710-1-AP

    IHC staining of mouse brown adipose using 32710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 32710-1-AP (Ucp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brown adipose using 32710-1-AP

    IHC staining of mouse brown adipose using 32710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 32710-1-AP (Ucp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UCP1 Polyclonal antibody

UCP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32710-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Mitochondrial brown fat uncoupling protein 1, Slc25a7, Solute carrier family 25 member 7, Thermogenin, Ucp

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brown adipose tissue
Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32710-1-AP targets UCP1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag38217

Product name: Recombinant mouse Ucp1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-178 aa of BC012701

Sequence: DSVQEYFSSGRETPASLGNKISAGLMTGGVAVFIGQPTEVVKVRMQAQSHLHGIKPRYTGTYNAYRVIATTESLSTLWKGTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uncoupling protein 1 (mitochondrial, proton carrier)
别名 Mitochondrial brown fat uncoupling protein 1, Slc25a7, Solute carrier family 25 member 7, Thermogenin, Ucp
观测分子量35 kDa
GenBank蛋白编号BC012701
基因名称 Ucp1
Gene ID (NCBI) 22227
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP12242
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

实验方案

Product Specific Protocols
IHC protocol for UCP1 antibody 32710-1-APDownload protocol
WB protocol for UCP1 antibody 32710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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