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验证数据展示

  • WB analysis using 32710-1-AP

    WB analysis using 32710-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32710-1-AP (Ucp1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brown adipose using 32710-1-AP

    IHC staining of mouse brown adipose using 32710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 32710-1-AP (Ucp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brown adipose using 32710-1-AP

    IHC staining of mouse brown adipose using 32710-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 32710-1-AP (Ucp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UCP1 Polyclonal antibody

UCP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32710-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Mitochondrial brown fat uncoupling protein 1, Slc25a7, Solute carrier family 25 member 7, Thermogenin, Ucp

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brown adipose tissue
Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32710-1-AP targets UCP1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag38217

Product name: Recombinant mouse Ucp1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-178 aa of BC012701

Sequence: DSVQEYFSSGRETPASLGNKISAGLMTGGVAVFIGQPTEVVKVRMQAQSHLHGIKPRYTGTYNAYRVIATTESLSTLWKGTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uncoupling protein 1 (mitochondrial, proton carrier)
别名 Mitochondrial brown fat uncoupling protein 1, Slc25a7, Solute carrier family 25 member 7, Thermogenin, Ucp
观测分子量35 kDa
GenBank蛋白编号BC012701
基因名称 Ucp1
Gene ID (NCBI) 22227
RRIDAB_3742718
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP12242
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

实验方案

Product Specific Protocols
IHC protocol for UCP1 antibody 32710-1-APDownload protocol
WB protocol for UCP1 antibody 32710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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