验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 4 publications below |
产品信息
21995-1-AP targets Tyrosinase in WB, IF, ELISA applications and shows reactivity with Rat, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Rat, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17314 Product name: Recombinant human Tyrosinase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-345 aa of BC027179 Sequence: HFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosinase (oculocutaneous albinism IA) |
| 别名 | LB24 AB, Monophenol monooxygenase, OCA1A, OCAIA, SHEP3, SK29 AB, Tumor rejection antigen AB, TYR, Tyrosinase |
| 计算分子量 | 529 aa, 60 kDa |
| 观测分子量 | 60 kDa, 70 kDa |
| GenBank蛋白编号 | BC027179 |
| 基因名称 | TYR |
| Gene ID (NCBI) | 7299 |
| RRID | AB_2918076 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14679 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Tyrosinase antibody 21995-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B EHMT2 promotes tumorigenesis in GNAQ/11-mutant uveal melanoma via ARHGAP29-mediated RhoA pathway | ||
Gene The degradation of TYR variants derived from Chinese OCA families is mediated by the ERAD and ERLAD pathway | ||
Arch Dermatol Res N-tert-Butylhydroxylamine promotes melanin production in oxidative stress conditions through the MITF signaling pathway | ||
J Cell Biol LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis |
