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验证数据展示

  • WB analysis using 26472-1-AP

    WB analysis using 26472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26472-1-AP (TRMT112 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 26472-1-AP

    IHC staining of human ovary tumor using 26472-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26472-1-AP (TRMT112 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRMT112/HSPC152 antibody included

TRMT112 Polyclonal antibody

TRMT112 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26472-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HSPC152, HSPC170, hypothetical protein HSPC152, TRM112 like protein, TRMT112

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRMT112/HSPC152 Ready-To-Use IHC Kit. TRMT112/HSPC152 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26472-1-AP targets TRMT112 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TRMT112 fusion protein Ag24126 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypothetical protein HSPC152
别名 HSPC152, HSPC170, hypothetical protein HSPC152, TRM112 like protein, TRMT112
观测分子量14 kDa
GenBank蛋白编号BC017172
基因名称 TRMT112
Gene ID (NCBI) 51504
RRIDAB_3085875
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRMT112 is a small evolutionarily conserved protein that acts as a co-factor and activator for different MTases involved in rRNA, tRNA and protein methylation. The TRMT112 interaction with 18S rRNA MTases WBSCR22 (BUD23, MERM1) and METTL5 is required for their metabolic stability and enzymatic activity in cells. (PMID: 34948388, PMID: 34948388)

实验方案

Product Specific Protocols
WB protocol for TRMT112 antibody 26472-1-APDownload protocol
IHC protocol for TRMT112 antibody 26472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma.

Authors - Baozhu Zhang
View Article