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验证数据展示

  • WB analysis using 68337-1-Ig

    WB analysis using 68337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68337-1-Ig

    WB analysis using 68337-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68337-1-Ig (THYN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of mouse testis using 68337-1-Ig

    IHC staining of mouse testis using 68337-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68337-1-Ig (THYN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

THYN1 Monoclonal antibody

THYN1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68337-1-Ig

产品说明书

CloneNo. 1E12G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68337-1-Ig targets THYN1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag8617

Product name: Recombinant human THYN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-166 aa of BC006978

Sequence: MSRPRKRLAGTSGSDKGLSGKRTKTENSGEALAKVEDSNPQKTSATKNCLKNLSSHWLMKSEPESRLEKGVDVKFSIEDLKAQPKQTTCWDGVRNYQARNFLRAMKLGEEAFFYHSNCKEPGIAGLMKIVKEAYPDHTQFEKNNPHYDPSSKEDNPKWSMKSLILF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thymocyte nuclear protein 1
别名 HSPC144, MDS012, My0054, MY105, THY28, THY28KD, thymocyte nuclear protein 1, Thymocyte protein Thy28, THYN1
计算分子量225aa,26 kDa; 166aa,19 kDa
观测分子量28 kDa
GenBank蛋白编号BC006978
基因名称 THYN1
Gene ID (NCBI) 29087
RRIDAB_3085061
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P016
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THYN1, also named as THY28, is a 28-32 kDa protein which located mainly in the nucleus. It appears to correlate with induction of apoptosis. (PMID:14601557)

实验方案

Product Specific Protocols
IHC protocol for THYN1 antibody 68337-1-IgDownload protocol
WB protocol for THYN1 antibody 68337-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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