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验证数据展示

  • WB analysis of HepG2 using 16604-1-AP

    WB analysis of HepG2 using 16604-1-AP

    WB result of TFB1M antibody (16604-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFB1M transfected HepG2 cells.

  • IP experiment of HepG2 using 16604-1-AP

    IP experiment of HepG2 using 16604-1-AP

    IP result of anti-TFB1M (IP:16604-1-AP, 4ug; Detection:16604-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

  • IF Staining of HepG2 using 16604-1-AP

    IF Staining of HepG2 using 16604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFB1M antibody (16604-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

TFB1M Polyclonal antibody

TFB1M Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 16604-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

CGI 75, CGI75, CGI-75, EC:2.1.1.-, h mtTFB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16604-1-AP targets TFB1M in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9785

Product name: Recombinant human TFB1M protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC017788

Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor B1, mitochondrial
别名 CGI 75, CGI75, CGI-75, EC:2.1.1.-, h mtTFB
计算分子量 346 aa, 40 kDa
观测分子量39 kDa
GenBank蛋白编号BC017788
基因名称 TFB1M
Gene ID (NCBI) 51106
RRIDAB_3085501
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVM0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)

实验方案

Product Specific Protocols
IF protocol for TFB1M antibody 16604-1-APDownload protocol
IP protocol for TFB1M antibody 16604-1-APDownload protocol
WB protocol for TFB1M antibody 16604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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