FLT3:42 nM

TCS 359，一种2-acylaminothiophene-3-carboxamide，是一种高效的FLT3抑制剂，其IC50为42 nM。它对MV4-11细胞增殖的抑制作用的IC50为340 nM，且对FLT3的选择性较其他激酶篇幅的高。[1]

DMSO:10.8 mg/mL (29.96 mM)

MV4-11 cells are plated at 10,000 cells per well in 100 μL of in RPMI media containing penn/strep, 10% FBS, and 0.2 ng/mL GM-CSF. Compound dilutions or 0.1% DMSO (vehicle control) is added to cells and the cells are allowed to grow for 72 h at standard cell growth conditions. To measure total cell growth, an equal volume of CellTiterGlo reagent is added to each well and luminescence is quantified. Total cell growth is quantified as the difference in luminescent counts of cell number at Day 0 compared to total cell number at Day 3 (72 h of growth and/or compound treatment). All IC50 values are calculated in GraphPadPrism using non-linear regression analysis with a multiparameter (variable slope) equation.(Only for Reference)