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验证数据展示

  • WB analysis using 30188-1-AP

    WB analysis using 30188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30188-1-AP

    IHC staining of mouse liver using 30188-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30188-1-AP

    IHC staining of mouse liver using 30188-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TASOR Polyclonal antibody

TASOR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30188-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C3orf63, FAM208A, se89 1, RAP140, KIAA1105

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30188-1-AP targets TASOR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32757

Product name: Recombinant human TASOR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1361-1512 aa of NM_001112736.1

Sequence: GKWQWKVHCKFQKKLKELGRLNAKALSLLTLLNVYQKKHLVEILSYHNCDSQTRNAPELDCLIRLQAQNIQQRHIVFLTEKNIKMLSSYTDNGIVVATAEDFMQNFKNLVGYHNSITEENLPQLGANENLESQSALLENDEKDEEDMSLDSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 3 open reading frame 63
别名 C3orf63, FAM208A, se89 1, RAP140, KIAA1105
计算分子量189 kDa
观测分子量189 kDa
GenBank蛋白编号NM_001112736.1
基因名称 TASOR
Gene ID (NCBI) 23272
RRIDAB_2935527
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK61
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TASOR, also known as FAM208A or C3orf63, is the central subunit of the human silencing hub (HUSH) complex. HUSH regulates deposition of the epigenetic mark H3K9me3. TASOR has a catalytically-inactive PARP domain which is necessary for targeted H3K9me3 deposition (PMID: 33009411). TASOR and the RNA deadenylase CCR4-NOT complex scaffold CNOT1 synergistically repress HIV proviral expression (PMID: 35013187). TASOR affects the genome stability during the cellular division and also interacts with methylation complex in adult tissues (PMID: 34272044).

实验方案

Product Specific Protocols
IHC protocol for TASOR antibody 30188-1-APDownload protocol
WB protocol for TASOR antibody 30188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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