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验证数据展示

  • IHC staining of human breast cancer using 10921-1-AP

    IHC staining of human breast cancer using 10921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10921-1-AP (TAF9B antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 10921-1-AP

    IP experiment of HeLa using 10921-1-AP

    IP result of anti-TAF9B (IP:10921-1-AP, 4ug; Detection:10921-1-AP 1:300) with HeLa cells lysate 4000ug.

TAF9B Polyclonal antibody

TAF9B Polyclonal Antibody for IHC, IP, ELISA
Cat No. 10921-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IP, ELISA

别名

DN 7, DN7, DN-7, Neuronal cell death-related protein 7, TAF9L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10921-1-AP targets TAF9B in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1362

Product name: Recombinant human TAF9B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC010350

Sequence: MESGKMAPPKNAPRDALVMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKPNVDADDVRLAIQCRADQSFTSPPPRDFLLDIARQKNQTPLPLIKPYAGPRLPPDRYCLTAPNYRLKSLIKKGPNQGRLVPRLSVGAVSSKPTTPTIATPQTVSVPNKVATPMSVTSQRFTVQIPPSQSTPVKPVPATTAVQNVLINPSMIGPKNILITTNMVSSQNTANEANPLKRKHEDDDDNDIM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
别名 DN 7, DN7, DN-7, Neuronal cell death-related protein 7, TAF9L
计算分子量 28 kDa
观测分子量 26 kDa
GenBank蛋白编号BC010350
基因名称 TAF9B
Gene ID (NCBI) 51616
RRIDAB_2199344
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7,transcription initiation factor TFIID subunit 9-like,transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.It's molecular mass is 31kd.

实验方案

Product Specific Protocols
IHC protocol for TAF9B antibody 10921-1-APDownload protocol
IP protocol for TAF9B antibody 10921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols