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TAF9B Polyclonal antibody

TAF9B Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP,ELISA

Conjugate

Unconjugated

Cat No : 10921-1-AP

Print datasheet

Synonyms

DN 7, DN7, TAF9B, TAF9L, TAFII31L, TFIID 31

验证数据展示

  • IHC staining of human breast cancer using 10921-1-AP

    IHC staining of human breast cancer using 10921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10921-1-AP (TAF9B antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 10921-1-AP

    IP experiment of HeLa using 10921-1-AP

    IP result of anti-TAF9B (IP:10921-1-AP, 4ug; Detection:10921-1-AP 1:300) with HeLa cells lysate 4000ug.

经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10921-1-AP targets TAF9B in IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP,ELISA
Tested Reactivity human, mouse, rat
Immunogen TAF9B fusion protein Ag1362 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
Synonyms DN 7, DN7, TAF9B, TAF9L, TAFII31L, TFIID 31
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC010350
Gene Symbol TAF9B
Gene ID (NCBI) 51616
RRIDAB_2199344
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7,transcription initiation factor TFIID subunit 9-like,transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.It's molecular mass is 31kd.

实验方案

Product Specific Protocols
IHC protocol for TAF9B antibody 10921-1-APDownload protocol
IP protocol for TAF9B antibody 10921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols