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TADA2L Polyclonal antibody

TADA2L Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11299-1-AP

Print datasheet

Synonyms

ADA2, ADA2 like protein, ADA2A, hADA2, KL04P, TADA2A, TADA2L, Transcriptional adapter 2 like

验证数据展示

  • WB analysis of mouse testis using 11299-1-AP

    WB analysis of mouse testis using 11299-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11299-1-AP (TADA2L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11299-1-AP

    IHC staining of human colon cancer using 11299-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11299-1-AP (TADA2L antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse testis using 11299-1-AP

    IP experiment of mouse testis using 11299-1-AP

    IP result of anti-TADA2L (IP:11299-1-AP, 4ug; Detection:11299-1-AP 1:300) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11299-1-AP targets TADA2L in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TADA2L fusion protein Ag1817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcriptional adaptor 2 (ADA2 homolog, yeast)-like
Synonyms ADA2, ADA2 like protein, ADA2A, hADA2, KL04P, TADA2A, TADA2L, Transcriptional adapter 2 like
Calculated Molecular Weight 51 aa, 5 kDa
Observed Molecular Weight 51.5 kDa
GenBank Accession NumberBC011753
Gene Symbol TADA2L
Gene ID (NCBI) 6871
RRIDAB_717042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TADA2A is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes [PMID:19103755].

实验方案

Product Specific Protocols
WB protocol for TADA2L antibody 11299-1-APDownload protocol
IHC protocol for TADA2L antibody 11299-1-APDownload protocol
IP protocol for TADA2L antibody 11299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

The double-histone-acetyltransferase complex ATAC is essential for mammalian development.

Authors - Guelman Sebastián S
View Article