验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11299-1-AP targets TADA2L in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1817 Product name: Recombinant human TADA2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC011753 Sequence: MDRLGSFSNDPSDKPPCRGCSSYLMEPYIKCAECGPPPFFLCLQCFTRGFEYKKHQSDHTYEIMTSDFPVLDPSWTAQEEMALLEAVMDCGFGNWQDVANQMCTKTKEECEKHYMKHFINNPLFASTLLNLKQAEEAKTADTAIPFHSTDDPPRPTFDSLLSRDMAGYMPARADFIEEFDNYAEWDLRDIDFVEDDSDILHALKMAVVDIYHSRLKERQRRKKIIRDHGLINLRKFQLMERRYPKEVQDLYETMRRFARIVGPVEHDKFIESHALEFELRREIKRLQEYRTAGITNFCSARTYDHLKKTREEERLKRTMLSEVLQYIQDSSACQQWLRRQADIDSGLSPSIP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcriptional adaptor 2 (ADA2 homolog, yeast)-like |
| 别名 | KL04P, hADA2, ADA2-like protein, ADA2A, ADA2 like protein |
| 计算分子量 | 51 aa, 5 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC011753 |
| 基因名称 | TADA2L |
| Gene ID (NCBI) | 6871 |
| RRID | AB_717042 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75478 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TADA2A is a subunit of the ADA2A-containing (ATAC) histone acetyltransferase complex, which with histone acetyltransferase activity on histones H3 and H4. It is required for the function of some acidic activation domains, which activate transcription from a distant site. It has a role in chromatin remodeling by binding at the linker region between neighbouring unclesomes of dinucleosomes [PMID:19103755].
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TADA2L antibody 11299-1-AP | Download protocol |
| IP protocol for TADA2L antibody 11299-1-AP | Download protocol |
| WB protocol for TADA2L antibody 11299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
