验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, A431 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20472-1-AP targets Synaptotagmin-4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14318 Product name: Recombinant human SYT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC036538 Sequence: MAPITTSREEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLKSSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | synaptotagmin IV |
| 别名 | KIAA1342, Synaptotagmin IV, SYT4, SytIV |
| 计算分子量 | 425 aa, 48 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC036538 |
| 基因名称 | Synaptotagmin-4 |
| Gene ID (NCBI) | 6860 |
| RRID | AB_10695633 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2B2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-4 is an atypical synaptic vesicle protein that is expressed in the dendrites of neurons. It mediates retrograde signals at the synapse and the release of BDNF, and regulates synaptic plasticity, motor learning, and memory.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptotagmin-4 antibody 20472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
