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验证数据展示

  • WB analysis using 26829-1-AP

    WB analysis using 26829-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26829-1-AP (SUSD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 26829-1-AP

    IHC staining of mouse cerebellum using 26829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26829-1-AP (SUSD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 26829-1-AP

    IF Staining of mouse cerebellum using 26829-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SUSD4 antibody (26829-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SUSD4 Polyclonal antibody

SUSD4 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 26829-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, ELISA

别名

UNQ196/PRO222, Sushi domain-containing protein 4, sushi domain containing 4, PRO222

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, TT cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26829-1-AP targets SUSD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 SUSD4 fusion protein Ag25346 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sushi domain containing 4
别名 UNQ196/PRO222, Sushi domain-containing protein 4, sushi domain containing 4, PRO222
计算分子量 490 aa, 54 kDa
观测分子量54 kDa
GenBank蛋白编号BC004888
基因名称 SUSD4
Gene ID (NCBI) 55061
RRIDAB_3669569
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VX71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SUSD4 (Sushi domain-containing protein 4) gene resides on chromosome 1q41 and encodes a 49-kD transmembrane protein containing four extracellular Sushi domain motifs. The Sushi domain, also known as the complement control protein domain, is commonly involved in protein-protein interactions. Many complement regulators are constructed with complement control protein domains. SUSD4 functions as a complement system regulator, as it has been shown to bind to the C1 complex and complement component C1q and block the activation of the complement cascade by interrupting the formation of C3 convertase. (PMID: 32179479)

实验方案

Product Specific Protocols
WB protocol for SUSD4 antibody 26829-1-APDownload protocol
IHC protocol for SUSD4 antibody 26829-1-APDownload protocol
IF protocol for SUSD4 antibody 26829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols