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SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12879-1-AP

Print datasheet

Synonyms

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1, sult2b1a, sult2b1b

验证数据展示

  • WB analysis of mouse skin using 12879-1-AP

    WB analysis of mouse skin using 12879-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12879-1-AP (SULT2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12879-1-AP

    IHC staining of human prostate cancer using 12879-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

  • IP experiment of mouse skin using 12879-1-AP

    IP experiment of mouse skin using 12879-1-AP

    IP result of anti-SULT2B1 (IP:12879-1-AP, 4ug; Detection:12879-1-AP 1:400) with mouse skin tissue lysate 3200ug.

经过测试的应用

Positive WB detected inmouse skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman prostate cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12879-1-AP targets SULT2B1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SULT2B1 fusion protein Ag3838 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfotransferase family, cytosolic, 2B, member 1
Synonyms Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1, sult2b1a, sult2b1b
Calculated Molecular Weight 365 aa, 41 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC034694
Gene Symbol SULT2B1
Gene ID (NCBI) 6820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of ULT2B1a and SULT2B1b.

发表文章

SpeciesApplicationTitle
humanWB

J Lipid Res

Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.

Authors - Ying Liu
View Article
mouseWB

Drug Metab Dispos

Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.

Authors - Takuyu Hashiguchi
View Article