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验证数据展示

  • IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • WB analysis using 27176-1-AP (same clone as 27176-1-PBS)

    WB analysis using 27176-1-AP (same clone as 27176-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

ST14 Polyclonal antibody, PBS Only

ST14 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27176-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EC:3.4.21.109, Matriptase, Membrane-type serine protease 1, MT-SP1, Prostamin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27176-1-PBS targets ST14 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26007

Product name: Recombinant human ST14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 301-539 aa of BC030532

Sequence: YNLTFHSSQNVLLITLITNTERRHPGFEATFFQLPRMSSCGGRLRKAQGTFNSPYYPGHYPPNIDCTWNIEVPNNQHVKVRFKFFYLLEPGVPAGTCPKDYVEINGEKYCGERSQFVVTSNSNKITVRFHSDQSYTDTGFLAEYLSYDSSDPCPGQFTCRTGRCIRKELRCDGWADCTDHSDELNCSCDAGHQFTCKNKFCKPLFWVCDSVNDCGDNSDEQGCSCPAQTFRCSNGKCLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppression of tumorigenicity 14 (colon carcinoma)
别名 EC:3.4.21.109, Matriptase, Membrane-type serine protease 1, MT-SP1, Prostamin
计算分子量 46 kDa
观测分子量 100 kDa
GenBank蛋白编号BC030532
基因名称 ST14
Gene ID (NCBI) 6768
RRIDAB_2880788
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Y6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

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