验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30232-1-AP targets SLC7A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | SLC7A2 fusion protein Ag31207 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 |
别名 | HCAT2, Cationic amino acid transporter 2, CAT-2 |
计算分子量 | 698 aa, 76 kDa |
观测分子量 | 76 kDa |
GenBank蛋白编号 | BC104905 |
基因名称 | SLC7A2 |
Gene ID (NCBI) | 6542 |
RRID | AB_3086271 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P52569 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Solute carrier family 7 member 2 (SLC7A2), also known as CAT2, is a member of the solute carrier superfamily. It functions as a cationic amino acid transporter, which can transport cationic amino acids (arginine, lysine and ornithine) into the cytosol and regulate inflammation (PMID: 35152203). It has been shown that lower SLC7A2 expression is associated with worse prognosis of ovarian cancer and hepatocellular carcinoma (PMID: 34108444; 32647070), and SLC7A2 deficiency in inflammatory bowel tissues increases the risk of inflammation-associated colon tumorigenesis (PMID: 30202097)
实验方案
Product Specific Protocols | |
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WB protocol for SLC7A2 antibody 30232-1-AP | Download protocol |
IHC protocol for SLC7A2 antibody 30232-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |